中药“健脑液”对培养神经细胞迟发性损伤的保护作用

The Protective Effect of Brain-Tonifying Liquid on Delayed Neuronal Damage in Vitro

以新生大鼠皮质神经细胞原代培养为实验对象，造成迟发性神经元损伤（ＤＮＤ）模型。在不同时程内，测试培养液中的细胞乳酸脱氢酶（ＬＤＨ）漏出量和用还原型尼克酰胺腺嘌呤二核苷磷酸脱氢酶（ＮＡＤＰＨ－ｄ）染色反应，观察培养细胞中一氧化氮合酶（ＮＯＳ）的表达水平。结果表明，缺血组在缺血与再灌流中，ＬＤＨ漏出量明显高于对照组，并随时间延长，差异越来越明显（Ｐ＜０．０５～０．０１）。ＮＯＳ阳性神经元数量在缺血时明显高于对照组（Ｐ＜０．０１），再灌流后早期ＮＯＳ表达仍然强烈，与对照组相比有显著差异（Ｐ＜０．０１）。从本实验细胞模型上，可以看到ＤＮＤ形成的机理与兴奋性氨基酸受体过度刺激造成第二信使效应过盛和自由基损伤两种因素关系密切。本实验应用以川芎、首乌、党参、葛根为主要成分的中药＂健脑液＂，在ＤＮＤ形成过程中，对细胞起到一定的保护作用。

This paper reported that the neuronal primary culture of cerebral cortex from newborn rats was used to make delayed neuronal damaged(DND) cellular model.The results indicated that,during the ischemia and reperfusion,there were more effusion of lactate dehydrogenase(LDH) in ischemia group than that in the control group, with the reperfusion time prolonged,both groups showed significant differences(P<0.05￣0.01). The number of nitric oxide synthase(NOS) positive neurons were larger than that of the control group(P<0.01) during the ischemia.After early phase in the reperfusion,the expression of NOS was still intense compared with the control group(P<0.01),but,after that,the level of NOS came down progressively.Whether NOS expression had timeliness? This question is worthy of further exploration.The results suggested that a close relationship exist between the formed mechanisms of DND and enlargement of second messagers responses induced by excitatory amino receptor abusive stimulation,and free radical damage in neuronal primary culture model.The medicinal herbs used in the experiment had some protective effect in the occurrence of DND.