肿瘤坏死因子对肿瘤细胞杀伤作用的病理研究

PATHOLOGICAL STUDY ON THE MECHANISM OF TUMOURSELECTIVE CYTOCIDAL-ACTION OF TUMOUR NECROSIS FACTOR

应用光镜和电镜观察了肿瘤坏死因子(TNF)杀伤人肝癌细胞系(SMMC-7721)的过程,同时测定了其DNA含量的变化,并观察了TNF对裸鼠人肝癌移植瘤的作用。结果表明,TNF与SMMC-7721细胞接触后,癌细胞膜系统先有变化,而胞核的变化出现在后。作者认为TNF选择性杀伤肿瘤细胞的机理可能是TNF与癌细胞膜上某种正常细胞不具有的物质发生作用,引起癌细胞的膜系统损伤,终致细胞死亡。

Using light microscope and electron microscope,we observed the morphological changes in the SMMC-7721 human hepatocellular carcinoma cell line after it was exposed to the rabbit tumor necrosis factor (TNF)and the cytocidal effect of TNF on the heterotransplanted human hepatocellular carcinoma (SMMC-7721 cell line).It was found that the changes occurred earlier in the cell membranes than in the nuclei during the course of TNF killing SMMC-7721 cell line and there was no special varieties around the necrotic area in the heterotransplanted human hepatocellular carcinoma in the nude mice as compared with that appearing in SMMC-7721 cell line,In addition, we detected the DNA contents of TNF treated SMMC-7721 cells and control ones, but there was no significant difference of the DNA contents between them.Ace of ding to the results of this study, we think that the mechanism by wluch TNF exerts its tumour-selective killing effect is probably as follows.TNF binds to a specific plasma membrane receptor and then disturbs synthesis or assembly of cell membrane components, leading the changes of cell structure(for example, swollen microvilli, a number of vesicles and small holes on the surface of tumor cells), and the lysis of tumor cells.