Epstein—Barr病毒基因组在鼻咽癌组织中转录的特征

CHARACTERIZATION OF EPSTEIN-BARR VIRUS GENOMETRANSCRIPTION IN NASOPHARYNGEAL CARCINOMA

对ＥＢ病毒基因在鼻咽癌活检组织细胞内的转录进行了较系统的探测。实验结果表明，ＥＢ病毒基因组在鼻咽癌活检组织中以附加体（Ｅｐｉｓｏｍｅ）形式存在，而其基因转录有如下特征：（１）ＥＢ病毒在所有鼻咽癌组织细胞中都表达ＥＢＮＡ－１，并且此基因转录产物由一个在ＢａｍＨＩ－Ｆ区的启动子（Ｆｐ）驱动；（２）；潜伏感染膜蛋白（Ｌａｔｅｎｔｍｅｍｂｒａｎｅｐｒｏｔｅｉｎ，ＬＭＰ和末端蛋白（Ｔｅｒｍｉｎａｌｐｒｏｔｅｉｎ，ＴＰ）的转录只在约５０％的活检组织中检测到，并且显示利用不同启动子的现象；（３）少部分的病例表达个别裂解基因；（４）在所有活检组织中，ＥＢ病毒基因组的ＢａｍＨＩ－Ａ区，表达一组功能未明的ｍＲＮＡ。通过对ＥＢ病毒基因在鼻咽癌细胞和其它受ＥＢ病毒感染的Ｂ淋巴细胞中表达情况的比较，本文对ＥＢ病毒基因在鼻咽癌组织细胞中的表达调控及其在癌变过程中的可能作用进行了讨论。

ranscription of Epstein-Barr virus genome in nasopharyngeal carcinoma wassystematically investigated and compared with that in other EBV-infected B cells Resultsshowed that EBV genome exists as episome in NPC cells and its transcription has thefollowing features: (1)EBNA-1 is expressed in all NPC cells and the EBNA-1 transcriptionis driven by a promoter(Fp) located in BamHI F region; (2)Only about 50% NPC tissuesshowed LMP and TP transcriptions and there wes differential promoter usage for LMP andTP genes; (3) Lytic gene expression was found in some of the NPC tissues; (4) A group oftranscripts with unknown function was found transcribed from BamHI Aregion in all theNPC tissues examined. Based on the data obtained,the expression and regulation of EBV genein NPC and its potential contribution to NPC were discussed.