摘要
Epstein-Barr病毒(EBV)进入鼻咽上皮细胞的途径,是人们研究EBV与鼻咽癌(NPC)的病因关系时所必须回答的一个问题。用抗EBV受体(EBVR/CR_2)单抗检测上皮细胞中该受体的表达已有报道,但对上皮细胞中EBVR/CR_2的基因结构仍需研究。我们采用PCR扩增和不对称PCR直接测序法,首次检测了10例NPC及3例正常人胚鼻咽上皮(Humanembryonicnasoparyngealepithelium,HENE)组织样本中EBVR/CR_2的EBV结合区的编码序列。这一片段的DNA序列测定结果显示,人NPC细胞和正常HENE细胞的EBVR/CR_2的EBV结合区的编码序列,与正常人B淋巴细胞的EBVR/CR_2的相应序列完全相同,提示EBV感染鼻咽上皮细胞可能与EBVR/CR_2基因的EBV结合区结构改变无直接关系。
ow Epstein-Barr virus enters nasopharyngeal epithelial cells is an essential question inunderstanding the pathogenitic relationship between EBV and nasopharyngeal carnicoma(NPC)Although there were some reports about the expression of EBV receptor (EBVR/CR_2) onepithelial cells using monoclonal antibodies against EBVR/CR_2, there were no reports on thestructural analysis of EBVR/CR_2 gene in epithelial cells. Here, we amplified the encodingregion of EBV binding site SCR_11 and SCR_2 (short consensus repeat) of EBVR/CR_2 by PCR(polymerase chain reaction) and performed DNA sequencing by asymmetric PCR method in 10cases of NPC samples and 3 cases of normal human embryonic-nasopharyngeal epithelial(HENE) cells. A 380 bp product was obtained after amplification. DNA sequencing of thisregion showed that the sequence of EBV binding site SCR_1 and SCR_2 of EBVR/CR_2 inhuman NPC cells and HENE cells was just the same as that of the nomal human Blymphocytes.The result suggests that there might be no direct relationship between EBV infection andthe change of EBV binding site of EBV receptor gene and this provides an experimentalevidenee for further studying the expression of EBV receptor.
出处
《病毒学报》
CAS
CSCD
北大核心
1995年第1期15-20,共6页
Chinese Journal of Virology
基金
国家八五攻关
CMB基金
关键词
EBVR/CR2
EB病毒
受体
鼻咽肿瘤
Nasopharyngeal carcinoma, Epstein-Barr Virus, EBV receptor/CR_2,Polymerase chain reaction, DNA sequencing