栗疫菌低毒力dsRNA与真菌病毒dsRNA的序列同源性

SEQUENCE HOMOLOGY dsRNA BETWEEN HYPOVIRULENT STRAINS OF CRYPHONECTRIA PARASITICA AND FUNGAL VIRUSES

以我国栗疫菌［Ｃｒｙｐｈｏｎｅｃｔｒｉａ（＝Ｅｎｄｏｔｈｉａ）ｐａｒａｓｉｔｉｃａ］低毒力菌株ＥｐＣ１４０（广西）的［γ─￣３２Ｐ］ＡＴＰ末端标记ｄｓＲＮＡ作探针，与５种真菌病毒ｄｓＲＮＡ进行分子杂交。探针可与紫孢侧耳病毒（Ｐｌｅｕｒｏｔｕｓｓｅｐｉｄｕｓｖｉｕｓ）和小麦全蚀菌病毒（Ｇａｅｕｍａｎ─ｎｏｍｙｃｅｓｇｒａｍｉｎｉｓ·ｖｉｒｕｓ）的ｄｓＲＮＡ杂交，但不能与黑曲霉病毒（Ａｓｐｅｒｇｉｌｌｕｓｎｉｇｅｒｖｉｒｕｓ）、产黄青霉病毒（Ｐｅｎｉｃｉｌｌｉｕｍｃｈｒｙｓｏｇｅｎｕｍｖｉｒｕｓ）和稻瘟菌病毒（Ｐｙｒｉｃｕｌａｒｉａｏｒｙｚａｅｖｉｒｕｓ）的ｄｓＲＮＡ杂交。当以低毒力菌株ＥｐＣ３２（云南）作探针时，结果相同。

Using γ-￣32P-ATP end-labelled fragments of denatured hypovirulence-associated dsRNAof chestnut blight fungus (Cryphonectria parasitica) EpC140as probe, dot blot hybridizationwas performed between EpC140 dsRNA and five fungal viraldsRNA. The dsRNA of EpC140hybridized well with viral dsRNA from Pleurotus sapidus(oyster mushroom) andGaeumannomyces graminis (wheat take-all disease fongus), but not with viral dsRNA fromPenicillium Chrysogenum (penicillin producer), Aspergillus nigerglucoamylase producer) andPyricularia oryzae (rice blast disease fungus). This result was confirmed by similar studieS inwhich another hypovirulent strain, EpC32, was used as probe.