摘要
用人工合成的丁型肝炎病毒抗原(HDV-Ag)肽建立了检测抗HDV-IgM抗体的ELISA方法,本法操作简便、快速,重复性好,特异性强,与抗HAV-IgM、抗Hk-IgM、抗HBs-IgM、抗HCV-IgI、抗CMV-IgM、抗RV-IgM、类风湿因子(RF)及抗核抗体(ANA)阳性血清均不起反应,且可被2-巯基乙醇阻断而不起反应。经初步临床应用,31例正常人血清抗HDV-IgM全部阴性,28例慢活肝患者检出率为32.1%(9/28),17例慢迁肝患者血清阳性率为11.8%(2/17)18例肝癌和肝硬化病人血清阳性率为22.2%(4/18)这三组病人与正常对照者相比较均有显著性差异(P<0.001)。此外,抗HDV-IgM阳性血清的ALT值均明显高于正常参考范围,提示在HDV感染过程中,患者肝细胞进一步受损。实验结果证明,抗HDV-IgM是诊断HDV感染的重要指标,对HDV感染早期诊断具有重要价值。
In this study,an HDV-IgM antibody detection assay (ELISA) was described by usingsynthetic peptide of HDV antigen, The assay was easy,rapid, reproducible and specific.Nocross-reaction was detected with IgM for hepatitis A,B(HBs,HBc),C and CMV ,RV(A),ANA,nor was there any interference by rheumatoid-factor.The results for sera of 31 normalpersons were all negative. But anti-HDV IgM was detected in 9 of 28 patients(9/28, 32.1%)with CAH, in 2 of 17 patients(2/17,11.8%) with CPH and in 4 of 18 patients(4/18, 22.2%)with liver cancer and cirrhosis(P<0.001).The ALT levels of the anti-HDV IgM positive serawere higher than those of negative sera, It is suggested that hepatic cells were injured while in-fected by HDV. These results demonstrated that identification of anti-HDV IgM is ofconsiderable significance in the early diagnosis and management of HDV infection.
出处
《病毒学报》
CAS
CSCD
北大核心
1995年第2期169-172,共4页
Chinese Journal of Virology