摘要
HAU3是寄主范围很广的放线菌噬菌体。Southern杂交实验表明,HAU3可以整合到吸水链霉菌应城变种10-22和变铅青链霉菌66的突变体ZX1的染色体中,形成溶原,其溶原菌自发释放HAU3,不受热激和紫外线照射的诱导。通过比较HAU3衍生噬粒pIJ8300的DNA酶切片段在加热前、后电泳带谱的区别,将HAU3的cos位点在pIJ8300的图谱上得到了定位。还利用Southern杂交的方法定位了HAU3与宿主形成溶原时附着位点(attP),并利用脉冲电泳技术定位了在变铅青链霉菌ZX7和吸水链霉菌应城变种10-22中形成溶原的附着位点(attB)。这些信息均有利于以HAU3为基础的载体的发展和优化。
opwood Actinophage HAU3 has a wide host range. Southern hybridization indicates that HAU3 DNA can lysogenize Streptomyces hygroscopicus 10-22 as well as Streptomvceslividans ZX7 by integrating into their chromosomes.No increase of phage production was ob-served after UV irradiation or incubdtion of HAU3 lysogenic spores. Comparison of the fragmentpatterns on agarose gels of annealed and heated digests of HAU3 derivative,plasmidpIJ8300 DNA (isolated from phage particles),allowed us to localize the HAU3 cos site on therestriction map of plasmid pIJ8300. In addition,attP site of HAU3 and attB site on thechromosome of Streptomyces lividans ZX7 and Streptomyces hygroscopicus var.yingchengensis10-22 strains have also been located by the use of pulsed-field gel electrophoresis and Sou-them hybridziation. These informations will be helpful for the development of HAU3 touseful gene cloning vector(s).
出处
《病毒学报》
CSCD
北大核心
1995年第2期163-168,共6页
Chinese Journal of Virology
基金
国家自然科学基金和国家教委留学服务中心资助