牛疱疹病毒Ⅰ型前早期基因BICPO的表达对牛免疫缺陷病毒LTR的反式激活作用

TRANSACTIVATION OF BOVINE IMMUNODEFICIENCY VIRUSLTR BY THE EXPRESSION OF BOVINE HERPES VIRUS ⅠIMMEDIATE EARLY GENE BICPO PROTEIN

由含有ＢＨＢＶ－１（ＢｏｖｉｎｅＨｅｒｐｅｓＶｉｒｕｓ－１）前早期基因的基因组片段亚克隆ＢＩＣＰＯ（ＢＨＶ－１ＩｎｆｅｃｔｅｄＣｅｌｌＰｒｏｔｅｉｎＯ）的ＤＮＡ序列至表达载体ｐＳＶＫ３，构建质粒ｐＳＶ２．９。将该质粒与ｐＢＬＴＲ－Ｌｕｃ共转染小牛肺细胞，检测转染细胞裂解物的荧光素酶活性，ＢＩＣＰＯ的表达产物可以显著地激活ＢＩＶＬＴＲ启动子控制下的荧光素酶基因的表达。根据ｐＳＶ２．９与含有ＢＩＶＬＴＲ不同区段缺失的质粒ｐＤ－３１９－Ｌｕｃ、ｐＤ－１１５－Ｌｕｃ、ｐＤ－５２－Ｌｕｃ共转染小牛肺细胞的实验结果，推测ＢＩＶＬＴＲ－３１９位上游区的ＤＮＡ序列影响ＢＩＣＰＯ基因产物对ＢＩＶＬＴＲ表达的激活作用。

ood BICPO coding sequencas were cloned into expression vector pSVK3 to producerecombinant plasmid pSV2.9. Cotiansfations of FBL cells with pSV2.9 and pBLTR-Lucshowed that BICPO p roduct could transactivate BIV LTR activity. To investigate thetransactivation mechanism, pSV2.9and pD-319-Luc,pD-115-Luc,pD-52-Luc whichwere BIV LTR partial deletion derivatives,were cotransfected separately into FBL cells. Theresults suggested that DNA sequenas upstream from-319 in BIV LTR may be responsible forBICPO protein transact ivation