用光生物素标记F20探针检测柑桔裂皮病类病毒

DETECTION OF CITRUS EXOCORTIS VIROID WITH PHOTOBIOTIN-LABELLED F20 PROBE

用光生物素标记Ｆ２０探针检测柑桔裂皮病类病毒陈纯贤，万蜀渊（武汉华中农业大学柑桔研究所４３００７０）关键词柑桔裂皮病类病毒，Ｆ２０探针，光生物素，斑点杂交核酸分子杂交技术因其灵敏度高、特异性强，也广泛用于病毒和类病毒的检测。最初，一般采用放射性同位素...

etection of citrus exocortis viroid(CEV)was conducted by using photobiotin -labelledF20 probe. Dot hybridization indicated hat F20 was very specific to CEV sequence, and thatthe highest dilution of crude CEV-nucleic acid extracts and the minimal amount of CEVdetected was about 1/100 and 1ng rtspectively. The concentration of photobiotiny-labelledprobe for dot hybridization should be at least 0.5μg/ml in general. The blocking reagent Tween 20 can effectively clean the membrane background and enhance its sensitivity.