摘要
水稻矮缩病毒基因组第九号片段的ｃＤＮＡ克隆及序列分析曲林，辛毅，朱玉贤，陈章良（北京大学生命科学学院，蛋白质工程及植物基因工程国家重点实验室北京１ｌｌ８７１）关键词水稻矮缩病毒，第九号片段，序列分析水稻矮缩病毒（ＲｉｃｅＤｗａｒｆＶｉｒｕｓ，ＲＤＶ）...
he cDNA of Rice Dwarf Virus (RDV)Fujian isolate genome segment S9was synthesized and amplified by RT-PCR. The PCR product was cloned and sequenced. The cloned fragment is 1,225bp in length, encodes a 39kD protein. This fragment has 93.9% homology in nucleotide sequence and 91.7% in amino acid sequence with RDV Japanese isolate S9. amino acid sequence comparison also showed that the N-terminal region of 39kD protein is highly conserved. It may suggest that the N-terminal region plays an important role in vivo in virus replication and encapsidation. Amino acid sequence comparison of proteins encoded by RDV S9 and WTV S11 also showed 32.9% homology.
出处
《病毒学报》
CAS
CSCD
北大核心
1995年第3期271-275,共5页
Chinese Journal of Virology
基金
国家科委863水稻抗病毒基因工程资助课题(863-101)
