人γ干扰素（IFN－γ）－表皮生长因子（EGF）融合蛋白的抗肿瘤细胞增殖作用与靶细胞的EGF受体有关

ANTIPROLIFERATIVE ACTIVITY ON A431 CELLS INDUCEDBY HUMAN IFN-γ-EGF3 FUSION PROTEIN ARE RELATEDTO ITS EPIDERMAL GROWTH FACTOR RECEPTORCOMPETTION

采用表皮生长因子（ＥＧＦ）受体丰富的Ａ４３１细胞进一步研究了人ＩＦＮ－γ－ＥＧＦ３融合蛋白￣〔１〕的抗细胞分裂活性，以及它和靶细胞ＥＧＦ受体的关系。结果表明，ＩＦＮ－γ－ＥＧＦ３融合蛋白的抗肿瘤细胞增殖作用明显高于其母体分子。￣１２５Ｉ－ＥＧＦ受体竞争抑制试验表明，ＩＦＮ－γ可与ＥＧＦ竞争Ａ４３１细胞的ＥＧＦ受体，而ＩＮＦ－γ－ＥＧＦ３融合蛋白的ＥＧＦ受体竞争抑制作用更为明显，说明人ＩＦＮ－γ和ＩＦＮ－γ－ＥＧＦ３融合蛋白的抗肿瘤细胞分裂活性，与靶细胞ＥＧＦ受体被竟争抑制密切相关。

he relationship between antiproliferative effect of human IFN-γ-EGF3 fusion proteinand the influence of EGF receptor binding activity has studied on A431 cell line.Theantiproliferative activity of human IFN-γ-EGF3 fusion protein was higher than that of itsparent IFN-γ.In the ￣125I-EGF receptor competition experiment,the inhibition of EGFreceptor binding capacity on the target cells was observed in the treatment of human IFN-γ,while enhancement of EGF receptor competiton was significant by the addition ofIFN-γ-EGF3 fusion protein.Our data suggest that the antiproliferative effects by INF-γand its fusion protein are closely related to their EGF receptor competition.