新疆幼畜和人非典型轮状病毒的调查和鉴定

INVESTIGATION AND IDENTIFICATION OF ATYPICALROTAVIRUS FROM ANIMALS AND HUMAN

自１９８４至１９９４年１０年间，在新疆采集了绵羊、山羊羔、仔猪、犊牛、骆驼羔和鸡的粪样１９５９份，以及住院腹泻婴幼儿、无症状新生儿和成人腹泻的粪样２５５份，共２２１４份。用ＰＡＧＥ法检测了这些粪样中的轮状病毒和非典型轮状病毒。按Ｓａｉｆ电泳型分组法，检出的各组轮状病毒是：Ａ组２７６份，在成人腹泻和驼羔中未检出；Ｂ组２６份，都是从羊羔和仔猪中检出；Ｃ组２２份，都是从仔猪中检出；Ｄ组２份，在鸡中检出；似Ｅ组１份，从仔猪中检出。取检出的各组非典型轮状病毒９株，即羊羔Ｂ组２株（ＫＢ－６３和ＬＢ－６０），仔猪Ｂ组４株（ＭＰＢ－１、２，ＳＰＢ－３、４），仔猪Ｃ组２株（ＳＰＣ－１１、ＺＰＣ－１２），仔猪似Ｅ组１株（ＭＰＢ－５），口服接种剥夺初乳的本种动物，接种后１２－１６小时动物都发生急性水样腹泻，并检出大量病毒。对这９株病毒和１株成人腹泻轮状病毒（ＡＤＲＶ），用ＥＬＩＳＡ和对流免疫电泳检测Ａ、Ｂ组组抗原，结果ＡＤＲＶ、羊羔Ｂ组、仔猪Ｂ组和似Ｅ组共８株病毒都只有Ｂ组组抗原。用Ａ、Ｂ组轮状病毒生物素核酸探针斑点杂交分别检测８株和１０株病毒，血清学检测为Ｂ组者都只与Ｂ组有杂交信号。Ｃ组的两株则既无Ａ、Ｂ组组抗原，也不与Ａ、Ｂ?

214 stool samples collected from animals and men during the last ten years (1984-1994)were examined for the presence of typical and atypical rotavirus by PAGE.56/1226 lamb and did,144/510 piglet,4/110 calf,2/108 chicken,26/68 infant and 46/113 the neonatal withoutdiarrhea were shown to contain group A rotavirus.No group A rotavirus was detectedin human adult and camel stool samples.16/1126 lamb and kid,10/510 piglet were shown tocontain group B rotavirus and no group B rotavirus was detected in other animals and men.GCRV was only detected in piglet(22/510).GDRV was only in chicken(2/108).GERV like wasonly in piglet(1/510). Nine strains of atypical rotavirus(ie.KB-63,LB-60,MPB-1,MPB-2,SPB-3,SPB-4,SPC-11, ZPB-12,piglet group E-like rotavirus)which belong to different rotavirus groupswere inoculated into neonatal animals deprived of clostrum.All animals developed acutewatery diarrhea and shed virus during 12-16 hours postinoculation.Group antigen of the nine strains as mentioned above and one strain of ADRV wereexamined with group A-ELISA,group B-ELISA and countercurrent immune electrophoresis(CIE). Dot hybridization of these strains were also carried out with photobiotin-labelednucleic acid probes for group A and group B rotavirus.The results indicated that ADRV,LB-60,KB-63,MPB-1,MPB-2,SPB-3,SPB-4 and the pigler group E-like rotaviruscontained group B rotavirus antigen only.They hybridiaed only with group B rotavirus nucleicacid probe.Group C rotavirus (i,e.SPC-11,ZPC-12) contained neithfer group A and group B rotavirus antigen nor hybridized with group A or group B nucleic acid probes.