EB病毒诱导永生化人上皮细胞发生恶性转化

EB VIRUS INDUCES MALIGNANT TRANSFORMATION OF IMMORTALIZED HUMAN EPITHELIAL CELLS

ＥＢ病毒诱导永生化人上皮细胞发生恶性转化李宝民，纪志武，刘振声，曾毅（中国预防医学科学院病毒学研究所，北京１０００５２）关键词ＥＢ病毒，促癌物，上皮细胞转化ＥＢ病毒与鼻咽癌有着密切关系￣［１，２］，已经证明ＥＢ病毒潜伏膜蛋白（ＬＭＰｌ）能引起大鼠细胞...

e established CR2-positive 293 cells, then, used EB virus to infect these cellsdirectly,In these cells EB virus can replicate and express EB viral antigens.EBvirus-infected epithelial cells grow in piles with multiple cellular layers and loss of contactinhibition. In soft agar culture,EB virus-infected cells together with TPA (12-0-tetardecanoyl-phorbol-13- acetate)treatment formed more and larger clones.When these cells were transplanted subcutaneously into nude mice,and treated with TPA,poorly differentiatedcarcinomas were induced.