影响桑树叶片培养不定芽形态分化主要因素的研究

STUDIES ON THE MAIN FACTORS INFLUENCING DIFFERENTIATION OF ADVENTITIOUS BUDS FROM MULBERRY LEAVES

以ＭＳ为基本培养基，探讨了植物激素、ｐＨ值、糖类、琼脂含量及桑树品种、外植体六因素对桑树叶片培养不定芽形态分化的影响，明确了植物激素是控制不定芽分化的决定因素，以ｐＨ５．６、琼脂６ｇ／Ｌ、葡萄糖２０ｇ／Ｌ为宜，桑树品种间不定芽分化率差异极显著，外植体以试管植株叶片分化率为高，田间植株叶片培养从桑树脱苞至停止生长前皆可进行。叶片不定芽分化率已达９６％。

Using MS as basic medium,the effects of phytohormones,pH,sugar,amount of a-gar,mulberry varieties and sources of explants on the differentiation of adventitiousbuds from mulberry leaves were studied.The results showed that phytohormones werethe most decisive factor.pH 5.6,agar content 6g/1 and concentration of glucose 20g/1were appropriate.The difference of differentiation rate was significant among differentmulberry varieties.The highest differentiation rate occurred to the leaves excised fromtube plants.Meanwhile the leaves of field plants could also be applied during a periodbetween bud exposing and growth stopping.A differentiation rate as high as 96%hasbeen obtained.