几种牧草和草坪草种子生活力四唑测定方法的研究

TETRAZOLIUM TEST METHOD OF SEED VIABILTY FLR SOME HERBAGES AND TURFGRASSES

对两种草坪草和六种牧草种子生活力四唑测定方法进行了研究，探讨了各种子适宜的预处理方法、四唑溶液浓度和染色时间。结果表明：除春箭舌豌豆种子外，其余种子在染色前均需水中浸渍或缓慢预湿及横切或纵切、针刺等预处理；在３０℃下测定的最佳条件为：假俭草、碱茅和籽粒苋种子用０．１％浓度，染色１４ｈ，狗牙根和串叶松香草种子用０．５％浓度，染色２２ｈ，春箭舌豌豆和湖南稷子种子用０．５％浓度，分别染色１４或６ｈ，小冠花种子用１．０％浓度，染色１４ｈ．

The tetrazolium test method of seed viability for two turfgrass and six herbage species were studi-ed. The optimum pretreatment concentration of tetrazolium solution and the staining time of each of thespecies were approached.The result showed that except the seeds of Vicia sativa, all the other ones neededpretreatment of soaking,slowly prewetting and latitudinal cutting and longitudinal cutting as well as pinprick-ing. At 30℃ the optimum condition of test was:the seeds of Eremochloa ophiuroides,puccinellia chinampoensis and Amaranthus paniculatus used 0.1% tetrazolium concentration,the time of staining was 14h;The seeds of Cyno-don dactylon and Siphium perfolium used 0.5% concentration,the time of staining was 22h:The seeds of Viciasativa and Echinochloa frumetacea used 0.5%,the staining time was 14h or 6h.The seed of Coranilla varia used 1.0%,the staining time was 14h.