犬急性出血性胰腺炎时Ⅱ型肺泡上皮细胞板层体体视学的研究

A STEREOLOGIC ANALYSIS OF LAMELLAR BODY WITHIN PULMONARY ALVEOLAR TYPE Ⅱ CELLS IN DOGS WITH ACUTE HEMORRHAGIC PANCREATITIS

本文报道了对犬实验性急性出血性胰腺炎(P组)与加用氯喹(PI组)时Ⅱ型肺泡上皮细胞的板层小体体视学形态定量的研究。结果发现:P组板层小体的体积密度(Vv),表面积密度(Sv)及数密度(Nv)分别较对照组下降29.06％,30.28％,及40.75％;其Sv及Nv分别较PI组减少23.8％和30.26％。结果提示:并发于急性出血性胰腺炎的肺损伤以肺泡表面活性物质系统受损为一重要的表现形式。板层小体数目显著减少,一定程度影响到肺泡的表面张力,从而造成肺不张。肺损伤与磷脂酶A_2的作用有关;氯喹在维持板层小体的数量方面可能有一定的作用。

A stereologic study of lamellar bodies within pulmonary alveolar type II cells in dogs with experimental acute hemorrhagic pancreatitis(group P)and in dogs inje- cted muscularly with choloroquine before inducing pancreatitis (group PI)was carried out.Our findings showed that volumerical density (Vv), surface density (Sv)and numerical density(Nv)of lamellar bodies were decreased by 29.06%,30.28% and 40.75% in the group P as compared with those in the control group (group N), respectively, and their Sv and Nv were decreased by 23.8% and 30.26% in comparison with those in the group PI.The results suggest that the lung injury resulting from acute hemorrhagic pancreatitis was characterized by alveolar surfactant System damage.Dramatical decrease in the number of the lamellar bodies affected the surface tension of alveoli in a certain degree, leading to atelectasis.There was a relationship between lung injury and the effect of PLA2(phospholipidase A2).Choloroquine might play a role in maintaining the number of lamellar bodies.