大鼠周血淋巴细胞的EGFR、EGF基因表达及ACTH—IR的观察

Observations on the EGFR-IR. EGF GeneExpression and ACTH-IR in Rat

本实验以２４只Ｗｉｓｔａｒ大鼠的密度梯度离心后的周血淋巴细胞作为观察对象，应用免疫组织化学方法显示ＥＧＦＲ—ＩＲ和ＡＣＴＨ—ＩＲ，用生物素标记的ｃＲＮＡ探针原位杂交显示ＥＧＦ的基因表达，此外对比观察了ＰＨＡ刺激转化的淋巴母细胞和未转化的淋巴细胞的ＡＣＴＨ－ＩＲ。结果见到淋巴细胞ＥＧＦＲ－ＩＲ呈现阳性、ＥＧＦ－ｍＲＶＡ信号呈蓝紫色颗粒布满胞质，提示淋巴细胞对ＥＧＦ可具有自分泌甚至旁分泌效应。ＡＣＴＨ－ＩＲ在未转化淋巴细胞呈弱阳性而转化的淋巴母细胞显著增强。

In this paper the EGFR-IR and EGF gene expressioll have been studioic by theimmullohistochemistry technique and in situ hybridization witch the salllples of rat circuirtinglymphocytes. Besides. the ACTH -IR was contrasted between transfomed and nontrans-formed lymphocytes. The results indicated that the EGFR-IR appeared positive reactloll andEGF-mRNA signal locallied at the lymphocytes. It suggests that there was EGF effect inlymphocytes via autocrine or paracrine rout. The ACTH-IR in the nontransformed lympto- cytes appeared week positivity. whereas, the ACTHI-IR was markedly enhaneed in the trans- formed lymphocytes.