兔角膜上皮细胞的培养及形态学观察

Culture and Morphologic Observations of the Rabbit Corneal Epithelial Cells

角膜上皮层是角膜组织的重要生物膜屏障,并为构成眼球光学屈光面的专一化组织,故临床医师对角膜上皮细胞层的病理损害历来十分关注。随着科学的进展,目前对角膜细胞病理的探讨已进入分子水平,故应用体外细胞培养作为研究手段正在应时开展。

This article reports that corneal epithelial cells were cultured by tissue block technique and observed by upsetting microscopy, ligh microscopy and electron microscopy. The results indicated that both corneal epithelial cells in culture and corneal epithelial cells in vivo are basically the same. The authors suggest that corneal epithelial cells in vivo can replace with corneal epithelial cells in culture in order to study physiology, histochemistry, biochemistry, immunity, etc.