玻璃体出血对视网膜组织的脂质过氧化损伤

Retinal Lipid Peroxidation Following Vitreous Hemorrhage in Rabbits

关于玻璃体出血是否引起视网膜损伤一直存在着争论。我们利用兔眼玻璃体出血模型,研究了视网膜脂质过氧化损伤。在玻璃体内注入0.2ml自体全血后,用硫代巴比妥酸荧光比色法测定视网膜内丙二醛含量。丙二醛是脂质过氧化的主要降解产物,在注血后10天达到0.166±0.087nmol/mg,明显高于对照眼(p<0.01)。透射电镜发现光感受器内、外节变性。本实验证实玻璃体出血对视网膜具有生化毒性。血红蛋白氧化和巨噬细胞产生的自由基作用,可能是富有脂质的视网膜损伤的重要原因,

It has still been debatable that blood in the vitreous is toxic to the retina. Lipid peroxidation of the retina in an experimental model of vitrious hemorrhage in rabbits was studied. Following in travitreal injection of 0.2ml autologous blood, the level of malondialdehyde (MDA)was measured by fluorescence spectrophotometry. The MDA reached a significant higher level (0.166±0.087nmol/mg) in experimental group than that of control eyes (P<0.01)in 10 days after blood injection. Degenerative changes of the inner or outer segments of photoreceptor cells were found in TEM.These results suggest that vitreous hemorrhage may be biochemically toxic to the retina.It appears that the lipid rich retina is highly susceptible toperoxidative damage by free radical which generated by hemoglobin oxidation and macrophages.