双歧杆菌分离、鉴定及其营养的研究

ISOLATION IDENTIFY AND RESEARCH IN NUTRITION OF BIFIDOBATERIUM

对从母乳和婴儿粪便中分离出的厌氧性杆菌进行双歧杆菌属、种鉴定，得到四株双歧杆菌，它们是：长双歧杆菌、青春双歧杆菌、短双歧杆菌和两歧双歧杆菌。用十一种不同培养基培养青春双歧杆菌，从中选出一种适宜做发酵用的种子培养基──ＢＳ培养基，选择五种物质为生长促进剂，进行牛乳发酵试验，其中大豆蛋白胨效果显著，菌浓度提高了２４倍，凝乳时间缩短了４８％，发酵酸度提高了１１％。

Idenfifying the anaerobe bacillus isolated from milk and night-soil of baby,we have obtaned four Bitidobacteriums,namely B. Longum,Badolescentis,B. Breve and B. bifidum,In order to find a seed-culture-BS culture which can be used to ferment,we have cutlured Badolescentis with elev-en different cutlures Conducting fermentation of milk test with five kinds of growth-promoter,it can be concluded that the effect of Soybean-peptoneis notable,the concentration of bacterium invreases 24times, the time of milk-coagulated can be Ahortened by 48 per,,and the acidity of fermentation in-creases 11 percent.