慢性肾衰血透患者脂质过氧化损伤与NK细胞活性的临床相关分析

Lipoperoxidation damage NK activity and clinic correlation in CRF patients with hemodialysis

本文测定２３例慢性肾衰血透患者血浆ＬＰＯ、ＳＯＤ、ＲＢＣ－ＬＰＯ及ＮＫ细胞活性。结果表明透前血浆ＬＰＯ、ＳＯＤ、ＲＢＣ－ＬＰＯ及ＮＫ细胞活性分别为１０．８５±２．１１ｎｍｏｌ／ｍｌ、４０．２４±８．０ｕ／ｍｌ、２３．９１±５．４９ｎｍｏｌ／ｇＨｂ和２９．９２±６．０８％（正常对照分别为６．５３±０．６５ｎｍｏｌ／ｍｌ、７９．２５±７．６１ｕ／ｍｌ、１６．３３±３．６１ｎｍｏｌ／ｇＨｂ和５１．２１±８．３３％）。透后血浆ＬＰＯ、ＳＯＤ分别为９．３８±１．５４ｎｍｏｌ／ｍｌ和５１．０７＋９．１６ｕ／ｍｌ，结果均与正常对照有显著差异；且透前、透后血浆ＬＰＯ、ＳＯＤ也有显著差异（Ｐ＜０．０１）。它们之间的相关分析表明血浆ＬＰＯ与ＢＵＮ、Ｃｒ呈正相关（ｒ＝０．３２５１Ｐ＜０．０５、ｒ＝０．４１９１Ｐ＜０．０１），与ＳＯＤ呈负相关（ｒ＝－０．５１１９Ｐ＜０．０１）；血浆ＳＯＤ与ＢＵＮ、Ｃｒ呈负相关（ｒ＝－０．５３５３、ｒ＝－０．４７６１Ｐ＜０．０１），ＮＫ细胞活性与ＲＢＣ－ＬＰＯ呈负相关（ｒ＝－０．４１３２Ｐ＜０．０５），与血浆ＬＰＯ、ＳＯＤ、ＢＵＮ、Ｃｒ无相关。上述表明慢性肾衰血透患者有脂质过氧化损伤，ＳＯＤ酶活性下?

Plasma lipid peroxidant(P-LPO),RBC-lipid peroxidant(RBC-LPO)levels and Plasma Superoxide Dismutase(P-SOD),NK activities in chronic renal failure(CRF) patients with hemodialysis(HD) were measured.The results showed that the patients with CRF before HD have significantly higher P-LPO(10.85±2.11nmol/ml),RBC-LPO(23.91±5.49nmol/gHb) and lower P-SOD(40.24±8.01ml),NK activities(29.92±6.08%) than that healthy controls(they were 6.53±0.65nmol/ml,16.33±3.61nmol/gHb,79.25±7.6l/ml and 51.21±8.33% respectively).After HD P-LPO is decreased and P-SOD is increased significantly(9.38±1.54nmol/ml and 51.07±9.16/ml respectively).Relative analysis between them revealed that P-LPO had a positive correlation with BUN and Cr(r=0.3251,P<0.05,r=0.4191 P<0.01) and a negative correlation with P-SOD(r=0.5119 P<0.01),P-SOD had a negative correlation with BUN and Cr(r=-0.5353,r=-0.4761 P<0.01),NK activity showed a negative correlation with RBC-LPO(r=-0.4132 P<0.05) but no correlation with P-LPO,P-SOD,BUN and Cr.It showed that there were lipoperoxidation damage lower P-SOD and NK activities in CRF patients with HD,iower NK activity was related to lipoperoxidation damage.