脑纤维组织细胞瘤免疫电镜研究

Immunoelectron Microscopical study of the fibrous histiocytoma of the brain

本文首次报告７例脑纤维组织细胞瘤免疫电镜结果。应用包埋后染色、胶体金Ａ蛋白法。选用５种免疫标记物（α_１ＡＴ、ａ_１ＡＣＴ、Ｖｉｍ．、Ｌｙｓ．、ＥＭＡ）。α_１ＡＴ、α_１ＡＣＴ及Ｖｉｍ。胶体金颗粒主要定位于组织细胞样细胞的胞浆内、溶酶体膜及粗面内质网；Ｌｙｓ．主要定位于组织细胞样细胞的溶酶体膜上；ＥＭＡ（２例阳性）定位于纤维母细胞样细胞浆内。本文提出脑的纤维组织细胞瘤具有更广泛的多向分化能力，尚可能向脑膜瘤细胞分化。

The results of 7 cases of the fibrous histiocytoma of the brain are reported with immunoelectron microscopy,using post-embedding staining and gold labeled protein A method for the first time. 5 kinds of the immunomarker were used. Electron microscopically ,a-l-antitrypsin ,a-l-antichy motrypsin and vimentin were chiefly located in endoplasma, lysosomal bodies and rough endoplasmic reticulums in the histiocyte-like cells,lysozymes were chiefly located in lysosomal bodies in the histiocyte-like cells. Epithelial membrane antigen (EMA) was positive in 2 cases. EMA was chiefly located in the plasmas of the fibroblast-like cells.It is proposed that the fibrous histiocytoma of the brain may differentiate to the epithelial cells or cells of meningiomas.It may have the ability of more broad multiple differentiation.