摘要
在实验控制条件下,采用5种蚊胃血血源鉴定方法,对淡色库蚊人工喂吸人血后60h内不同消化阶段(Sella期)的胃血血源检测结果作对比分析,结果表明酶联直接法、斑点法(A法和B法)具有较高的灵敏度和特异性。并对中华按蚊、淡色库蚊和埃及伊蚊等不同蚊种,人、猪、鸡、狗、牛等不同宿主血源,以及人猪和人猪鸡混合血的血源鉴定效果作了进一步探讨。
A study under laboratory control was conducted by direct-ELISA, dot-ELISA (A, B), coagglutination test and precipitin test for the mosquito bloodmeals identification of Culex Pipiens pollens at different intervals from 1 to 60 h after engorging on the blood of human, pig, chicken, dog and cow. Results indicated that direct-ELISA and dot-ELISA were of higher sensitivity and specificity than others. Dot-ELISA is an ideal method for the identification of mosquito bloodmeals because of its simplicity and rapidity, some factors such as mosquito species (Anopheles sinensis Aedes aegypti and Cu. pipiens pattern), host blood sources and mixed bloodmeals which affect the results are also discussed.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1989年第6期538-543,共6页
Academic Journal of Second Military Medical University
关键词
酶联免疫吸附
蚊胃血
鉴定
direct-ELISA
eoagglutination test
preeipitin test
mosquito bloodmeal identification
