抗细菌脂多糖核心糖脂域单克隆抗体在实验性菌血症中的保护作用

Protective Effects of Monoclonal Antibodies to Core Glycolipid of Bacterial Lipopolysaccharides in Experimental Bacteremia

以小鼠菌血症模型对抗核心糖脂域ＭｃＡｂ（ＥＬ１、ＥＬ３和３Ｈ４）的免疫保护的效果进行了研究。结果表明，（１）３株ＭｃＡｂ能显著提高受Ｅ．ｃｏｌｉ攻击小鼠的存活率（Ｐ＜０．０５）；（２）ＥＬ３和３Ｈ４还分别有降低３ＬＤ５０绿脓杆菌和５ＬＤ５０鼠伤寒沙门氏菌感染小鼠病死率的作用（Ｐ＜０．０５）；（３）ＥＬ３与３Ｈ４对受５ＬＤ５０Ｅ．ｃｏｌｉ和１０ＬＤ５０绿脓杆菌攻击小鼠有一定的协同保护作用；（４）在ＥＬ１预防性保护试验中发现，提前４周注入ＥＬ１，并于攻菌前１周加强一次，ＥＬ１可明显提高５ＬＤ５０Ｅ．ｃｏｌｉＪ５株攻击小鼠的存活率（Ｐ＜０．０５）。提示ＥＬ３和３Ｈ４对细菌感染小鼠具有一定的交叉保护作用。

The immunoprotective effectS of anti-CGL McAbs(EL1,EL3 and 3H4) in expertriental bacteremia of mice were studied.The results demonstrated that:(1) The survival rates of mice challenged with E.coli strain were significantly higher in treated groups of three anti-CGL McAbs than those in controls(P<0.05);(2) EL3 and 3H4 also presented the effective protection for mice challenged with 3 LD50 P.aeruginosa and 5 LD50 S.Typhimurium respectively(P<0.05);(3) the synergic efficacies of these two anti-CGL McAbs combination therapy on reducing mortality of mice were demonstrated respectively in 5 LD50 E. coli and 10 LD50 P.aeruginosa bacteremia models;(4) a new experiment of prophylactic treatment showed that the survival rate of mice pretreated with EL1 four weeks befote and retreated with the same McAb one week before 5 LD50 E.coli J5 strain challenge was found tO be significantly increased,compared with parallel control mice pretreated with nonrelatedMcAb(M11)(P<0.05).