深低温保存小鼠骨髓细胞及其移植效果

CRYOPRESERVATION OF MURINE BONE MARROW CELLS AND THEIR TRANSPLANTATION

小鼠骨髓细胞经程序降温-深低温保存后,给经致死剂量照射小鼠进行同种骨髓移植治疗造血障碍。实验用ICR.SMMC/C及昆明种小鼠219只,体重26～33g 8～9周龄,冻存骨髓细胞融解后,台盼蓝拒染率为70％～80％,CFU-GM,CFU-S测试均与新鲜骨髓结果相似。同种移植后动物活存率达43.6％,与新鲜骨髓无明显差别。冻存骨髓细胞移植加从小牛脾脏提取的抑素(Chalone)体内处理,动物活存率为42.5％,而Chalone体外处理活存只有25.0％,Chalone在冻存骨髓细胞移植中的作用尚待进一步实验。

Murine bone marrow cells were cryopreserved in liquid nitrogen with programmable freezing system as a source of hematopoietic cells to transplant into irradiated recipients for the treatment of hematopoietic failure. The results showed that CFU-GM in graft was slightly affected by freezing with a recovery of 75.7%, but CFU-S which act as major role in hematopoietic recons-titution was not obviously injuried (recovery of 126.7%). In the transplantation study, the frozen-thawed bone marrow cells gained survival rate of 43.6% which was slightly higher than obtained by fresh cells, suggesting that cryopreserved bone marrow cells still have intact hematopoietic reconstructive ability. For the purpose of preventing graft-versus-host disease (GVHD), chalone, an extract from calf spleen, was used in our studies and inhibited the early GVHD effectively.