三种免疫学方法检测抗班氏丝虫IgG的比较

Comparison of Three Immunologic Assay for Detecting Specific IgG against Filaria bancrofti in Cases with Bancroftian Microfilaremia

用酶联免疫吸附试验（ＥＬＩＳＡ）、斑点酶联免疫吸附试验（Ｄｏｔ一ＥＬＩＳＡ）和斑点免疫金银染色（Ｄｏｔ一ＩＧＳＳ）同步检测５０份班氏微丝蚴血症者血清中抗丝虫ＩｇＧ。ＥＬＩＳＡ、Ｄｏｔ一ＥＬＩＳＡ和Ｄｏｔ一ＩＧＳＳ检测抗体阳性率分别为８６％（４３／５０）、９０％（４５／５０）和１００％（５０／５０）。ＥＬＩＳＡ和Ｄｏｔ一ＥＬＩＳＡ联合应用阳性率为９６％（４８／５０）。ＥＬＩＳＡ与Ｄｏｔ一ＩＧＳＳ检测微丝蚴血症者抗体阳性率有显著性差异（Ｐ＜０．０５），抗体阳性率在ＥＬＩＳＡ与Ｄｏｔ一ＥＬＩＳＡ、Ｄｏｔ一ＥＬＩＩＳＡ与Ｄｏｔ一ＩＧＳＳ之间则无显著性差异。三种方法所检测的抗体强度与外周血中微丝蚴密度无相关关系。

Enzyme一linked immunosorbent assay(ELISA ),dot一ELISA and Dot一immuno gold silverstaining(Dot一IGSS) were used to detect the specific IgG against filaria in sera collected from 50 bancroftianmicrofilaremia cases simultaneously. The positive rate was 86%(43/50), 90%(45/5) and l00%(50/50)withELISA,Dot一ELISA and Dot一IGSS respectively. The positive rate was 96%(48/50) when ELISA and Doot一ELISA were combined. There was a significant difference between the results detected with Dot-IGSS andELISA(P<0. 05) and there were no statistically significant differences between ELISA and Dot一ELISA andbetween Dot一ELISA and Dot-IGSS respectively. There was no correlation between the microfilaria densityand the specific antibody level detected by the three serologic tests.