福建三种家鼠寄生蚤基因组DNA限制性内切酶酶切初步分析

Preliminary Studies on Restriction Enzymic Analysis of Genomic DNA in Three Species of Domestic Rat Flea of Fujian

从实验室饲养的３种家鼠寄生蚤，印鼠客蚤（Ｘｅｎｏｐｓｙｌｌａｃｈｅｏｐｉｓ）、缓慢细蚤（Ｌｅｐｔｏｐｓｙｌｌａｓｅｇｎｉｓ）和不等单蚤（Ｍｏｎｏｐｓｙｌｌｕｓａｎｉｓｕｓ）制备出基因组ＤＮＡ，用８种识别不同位点的限制性内切酶ＥｃｏＲＩ，ＨｉｎｄⅢ，ＰａｓｔＩ，ＳａｌＩ，ＢａｍＨＩ，ＨｐａＩ，ＰｖｕⅡ，ＢｇｌＩ对蚤基因组ＤＮＡ进行单酶完全酶切分析。印鼠客蚤基因组ＤＮＡ经ＥｃｏＲＩ酶解电泳后，见６条带，分别为３．２２ｋｂ，２．９３ｋｂ，２．２０ｋｂ，１．５７ｋｂ，１．５３ｋｂ，１．４８ｋｂ，缓慢细蚤与不等单蚤仅见一条，分别为５．７９ｋｂ和７．０１ｋｂ。经ＢａｍＨＩ酶切，印鼠客蚤ＤＮＡ见１条２．５４ｋｂ的带，缓慢细蚤与不等单蚤分别有２条，各为７．３５ｋｂ，０．５９ｋｂ和１２．２９ｋｂ，０。６２ｋｂ。另外，印鼠客蚤经ＨｐａＩ酶切后，还有２条分子量很相近的ＤＮＡ带型，分别为１．５６ｋｂ，１．５４ｋｂ。此外，３种蚤在其他内切酶酶切产物中未显肉眼可见的电泳带型。酶切结果显示缓慢细蚤与不等单蚤很相似，这２种蚤具有较大的同源性，说明其亲缘关系较近。印鼠客蚤酶切产物与另外２种蚤差别很大，提示它在蚤的进化过种中较早分化出来，故与?

Genomic DNA from three species of domestic rat flea,Leptosylla segnis,Mon0psyllus anisus and xenopsylla cheopis,were prepared,then digested with eight restriction enzymes. In digestion with EcoR I and electrophoresis,the genomic DNA of X.cheopis showed six bands(3. 22 kb,2. 93 kb,2.20 kb,1.57 kb,1.53 kb,1.48 kb),while only one band in L.segnis(5. 79 kb)and in M.anisus(7. 01 kb).In the analysis of BamH I,the X.cheopis genomic DNA gave one band(2.54 kb),while L.segnis(7.35 kb,0.59 kb) and M.anisus (12.29 kb,0.62 kb)gave two bands,respectively.On the other hand,X. cheopis genomic DNA showed two bands( 1.56 kb,1.54 kb )in the HpaI experiment,but no bands in the other two species of fleas. The re-mained REs showed no visible bands in any of the three species’ DNA. The results of REs digestion in L. seg-nis and M. anisus were similar to each other,implicating that these two species were in close relatives;howev-e,the results of REs’digestion in X cheopis were much different from the other two species,suggesting it an earlier differentiation in phylogeny