大白鼠肠系膜上动脉夹闭休克时SOD抗脂质过氧化损伤的研究

Oxygen -derived Free Radicals Induced Cellular Injury in Superior Mesenteric Artery Occlusion Shock : Protective Effect of Super oxide Dismutase ( SOD)

本实验探讨大白鼠肠系膜上动脉夹闭休克时输注SOD的抗脂质过氧化作用和细胞保护效应。松夹1h,实验组肠和心肌MDA较对照组明显升高(P<0.05),而SOD治疗组肠和心肌MDA明显低于实验组(P<0.05),与对照组无显著差异(P>0.05)。松夹2h,实验组肠、心、肝、肺MDA均明显高于对照组(P<0.5～0.01),而SOD治疗组肠、心、肺MDA仍低于实验组(P<0.05),但较对照组明显升高(P<0.05)。此外,SOD治疗组血浆β-葡萄糖醛酸酶和酸性磷酸酶明显低于实验组,动物存活时间明显延长。

Intraaterial injection of superoxide dismutase (SOD)15000U/kg 15 min before releasing the obstruction is given . 35 rats are divided into 3 groups : group 1, 7 for control; group 2,14 for releasing the obstruction for 1 h ; in which (7 as 2A , 7 as 2B ) 2B are given SOD ; group 3 , 14 for releasing the obstruction for 2 h , in which (7 as 3a , 7 as 3B ) 3B given SOD . The results show : the content of malondialdehyde (MDA) in bowel , and heart tissue in 2A increases 66% and 55% (p<0.05) compared to group 1 , content of MDA in 2B in the same kind of tissue decrease 42% and 41% (p<0. 05) vis 2A , and no significant change (p> 05) vis group 1 ; contents of MDA in bowel, heart, liver , and lung tissues in 3A increase 126% , 69%,36%, and 28% (p<0.01 ,0.05) vis group 1, but in 3B they are lower than 3A(p<0.05), higher than group 1 (p<0.05). Plasma β-glucuronidase and acid phosphatase in 2B and 3B are much lower than 2A and 3A . The survival time of shocked animals treated with SOD is markedly prolonged. The results suggest that administration of SOD can protect cells against the peroxidative tissue damage produced by oxygen - derived free radicals , depress the release of lysosome enzymes and prolong the survival time of shocked animals .