多糖多相脂质体的物理性质与稳定性研究

STUDIES ON THE STABILITY AND SOME OTHER IMPORTANT PHYSICAL PROPERTIES OF POLYSACCHARIDES POLYPHASE LIPOSOME (PPL)

本文主要研究多糖多相脂质体的物理性质——多相脂质体加热灭菌前后的形状、结构、粒度分布变化以及对药物丝裂霉素的包封率和聚结稳定性。实验证明:多糖多相脂质体的热稳定性好。经100℃60 min灭菌可保持完整多相脂质体形态、均匀的粒径与稳定的包封率。用Coulter计数仪测定了贮存过程中聚结成人粒子的变化规律。测得其聚结活化能E=99.992 kJmol^(-1),是比较稳定的多相脂质体。

The stability and some other important physical properties of PPL were investigated by means of electron microscopy and 'MPV-TAS experiments.The results are rated as follows:Good integrity of liposome homogeneity of its particle size were reached, and at 100℃ PPL was found to undergo sterilization without causing changes of its, shape and encapsulation efficiency for 1 h. Also, the distribution of PPL particle size and the regularity of the change of PPL particle in size were determined by Coulter counter.Using this method, the kinetic equation of coalescence of polyphase liposome and the activation energy for coalescence were obtained, and through these results. the stability of PPL is elucidated.