阿斯匹林与烟酸联合应用时血药浓度测定

DETERMINATION OF ASPIRIN AND NICOTINIC ACID IN PLASMA WHEN USED IN COMBINATION

阿斯匹林与烟酸给家兔同时ⅳ后,采用新建立的溶剂萃取,分光光度法和Carlson法分别测定两药血浆浓度。结果用微机分析拟合曲线,符合二室开放模型。两药联合应用后,烟酸T_(1/2)β(2.3374 h)和AUC(361.63μg·h/ml)较单用炳酸时T_(1/2)β(0.9846h)和AUC(157.71μg·h/ml),差异非常显著(P<0.01)。其它参数在两种情况下均无显著差别。

An ultraviolet spectrophotometric method has been developed for indirect determination of aspirin (ASA)using the difference between total salicylic acid and free salicylic acid in rabbit plasma. Recoveries of salicylic acid were found to be above 98.4% and the method was linear from 8～1601μg with a correlation coefficient of 0.9999. The coefficient of variation was less than 3.85%.Using this method for ASA and Carlson's mcthod for nicotinic acid (NA), the plasma concentrations of these two orugs were cetermired when they were used iv alone or in combination in rabbits. The results analysed by a micro-computer were fitted to a two compartment open model with firstorder elimination. Among the pharmacekintic parameters of NS:, T_(1/2)(β) and AUC^(0-∞) for the group (n=6) treated with NA 50 mg/kg in combination with ASA 50 mg/kg were 2.3374 h and 361.63 μg.h/ml, respectively, both significantly different from those for the group (n= 6) treated with NA 50 mg/kg alone which were 0.9846 h and 157.71μg·h/ml,resFectively(p<0.01). For ASA, no significant differences were demonstrated. The mechanism and significance of the prolonged T(1/2)(β) of NA were discussed.