香荚兰素对抗大鼠杏仁核点燃效应

THE EFFECT OF VANILLIN ON THE FULLY AMYGDALA-KINDLED SEIZURES IN THE RAT

用大鼠杏仁核点燃模型研究了香荚兰素的抗癫痫作用。每日一次低电流刺激杏仁基底外侧核可在第15天使大鼠产生典型的五期反应,刺激后放电显著延长。香荚兰素在不产生中枢抑制作用的剂量即可抑制点燃效应的五期反应,ED_(50)为286 mg/kg(ip),同时使刺激后放电时程比对照组下降28.6%。苯妥英钠抑制五期反应的ED_(50)为56mg/kg,也能明显缩短刺激后放电时程。本文提供了一种新型慢性实验性癲痫模型。

The anticonvulsive action of vanillin, an antiepileptic agent structurally related to vanillyl alcohol isolated from Gastrodia elata Blume, on the fully amygdala-kindled seizures was investigated. Fully kindled seizures were produced by repeated application of low intensity electrical stimulation to the basolateral amygdala once per day. Fifteen consecutive stimulations were needed to reach the first stage 5 seizures at which animals showed behavioral convulsions and elcctroencephalographic abnormalities. The stage 5 seizures were suppressed by vanillin ip 1 h before stimulation with the ED_(50)of 286 mg/ kg. Meanwhile, the epileptic afterdischarge duration was significantly shortened. Phenytoin at a nontoxic dose 50 mg/kg ip obviously reduced the stage 5 seizures. The present study provides a very useful experimental model of chronic epilepsy to detect anticonvulsants.