高效液相色谱法同时测定卡马西平及其活性代谢物的血浓度

SIMULTANEOUS DETERMINATION OF CARBAMAZEPINE AND ITS ACTIVE METABOLITE IN SERUM BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

卡马西平(carbamazepine,以下简称CBZ),又名痛痉宁、酰胺咪嗪(结构式见图1)是一种新型抗癫痫药。以后又发现该药对治疗三叉神经痛、尿崩症及躁狂症亦有较好的疗效。但该药的不良反应如复视、共济失调、眩晕及消化道症状颇为严重,据报道,至少有25%的患者在用药期间发生各种不良反应。

A rapid, sensitive and simple to operate high performance liquid chromatographic method for the simultaneous determination of carbamazepine (CBZ) and its metabolite (10,11-epoxide carbamazepine,ECBZ) in serum has been developed. The sample was extracted with ethyl acetate. The extract was evaporated to dryness and taken up with the mobile phase. Separation of CBZ and ECBZ was achieved by reversed phase chromatography using a mobile phase consisting of methanol-water (1:1) at flow rate of 0.8 ml/min on a 5 μm YWG C-18 column. Eluent was monitored at 214 nm.The method has a good linearity. The recoveries of CBZ and ECBZ were found to be 99.7%±2.45 and 97.3%±4. 20 respectively. Precision studies for both within day and day-to-day at different concentrations provided CV values of less than 6%. Some commonly used anticonvulsants can be determined in the same procedure without interference. This method well adapted to the therapeutic monitoring of CBZ treated patients, as well as for pharmacokinetic studies.