薄层色谱溶剂系统的最优化方法

METHOD OF OPTIMIZATION OF THE SOLVENT SYSTEM FOR THIN LAYER CHROMATOGRAPHY

本文以模糊聚类分析法对81种常用溶剂进行分类,并根据最相邻原理从中优选出二十种色谱分析常用溶剂。再据此结果,以均匀设计试验法和最优化方法——单纯形法为手段,进行溶剂系统的最优化设计。并提出一个改良的色谱优化函数。经初步验证,这是一种比较有效与实用的系统化方法,尚未见国内外报道。本法不仅适用于薄层色谱,也适用于纸色谱及柱型液相色谱。

In this article, we analized 81 usual solvents with Fuzzy group analytical method by a computer at first, and selected 20 better solvents for TLC. Then optimized the solvent system for TLC by means of uniform design test and optimization method——simplex method. We also worked out a modified chromatographic optimization function. This systematized method has been shown to be effective and practical in our experiments. It is not only fit to TLC, but also fit to paper chromatography and liquid chromatography. So far, no similar report has been found in the literature.