甘草类黄酮对脂质过氧化和活性氧自由基的作用

EFFECTS OF GLYCYRRHIZA FLAVONOID ON LIPID PEROXIDATION AND ACTIVE OXYGEN RADICALS

本文用比色法测定过氧化脂质研究甘草类黄酮(FG)的抗脂质过氧化作用,并用化学发光法和自旋捕集技术检测该药对O_2^(?)和OH~·的清除作用。体外实验表明:FG 2.8～25μg/ml可明显抑制小鼠肝匀浆在振荡温育条件下引起的丙二醛升高;FG 0.265～26.5μg/ml或2.58～258 μg/ml分别对碱性二甲基亚砜或黄嘌呤/黄嘌呤氧化酶体系生成的O_2^(?)有显著清除作用;以上作用呈浓度依赖性变化。FG 144 μg/ml或258 μg/ml分别对PMA刺激多形核白细胞释放的O_2^(?)及OH~·或Fenton's反应生成的OH~·有明显清除作用。实验证明FG有抗氧化作用。

The antiperoxidant activity of glycyrrhiza flavonoid (F G) was studied by using colorimetric estimation of lipid peroxide(MDA) formation. The scavenging effects of FG on O_2- and OH^- was investigated by using chemiluminescence method and spin trapping technique in different systems. The results were as follows. FG 2.8～25μg/ml effectively inhibited MDA formation induced by incubating mice liver homogenateat 37℃ for 1h; FG 0.265～26.5μtg/ml or 2.58～258μg/ml was shown to markedly scavenge O_2 in alkaline/DMSO or xanthine/xanthine oxidase systems, respectively.in a concentration-dependent manner. FG 144μg/ml or 258μg/ml also significantly scavenged the active oxygen radicals produced by PMN stimulation with PMA or OH^- produced in Fenton's reaction respectively. The results suggest that FG may be used as an antioxidant.