高效薄层扫描法测定166种杜鹃中6种黄酮类成分的含量

HPTLC SCANNING DETERMINATION OF 6 FLAVONOIDS IN 166 RHODODENDRON SPECIES

在高效硅胶薄层板上以石油醚(bp60～90℃)—乙醚—甲酸(62:31:7)的上层(展开剂Ⅰ)分离杜鹃素、山奈素和槲皮素;扫描测定后再次展开,以氯仿—甲醇—水(7:3:1)的下层—甲酸(7:0.5)(展开剂Ⅱ)分离蓼属甙、槲皮甙干口金丝桃甙。用瑞士Camag 76510型单波长薄层扫描仪进行直线扫描,测定波长366nm,用美国Hewlett Packard 3390 A型积分仪测出峰面积。测定了166种杜鹃叶中上述6种黄酮类成分的含量。

This paper reports a quantitative determination of the contents of flavonoids in Rhododendron leaves by HPTLC scanning method. The methanol extract of each sample was spotted on a HPTLC silica gel plate (E.Merck F_(254))alongside with standard substances. Using the upper layer of petroleum ether(60～90℃)-ether-formic acid (62: 31:7) as developing solvent Ⅰ, farrerol, kaempferol and quercetin were well separated. The spots were determined by a single wavelength (366 nm) TLC-scanner, Camag model 76510. The same plate was then further developed by developing solvent Ⅱ.7 ml of the lower layer of chloroform-methanol-water (7: 3:1) plus 0.5 ml of formic acid to separate polystachoside, quercitrin and hyperoside. The densitometric determination of each spot was also carried out with the same scanner.The contents of 6 flavonoids in the leaves of 166 Rhododendron species were thus determined.