非何杰金淋巴瘤核仁组成区蛋白的研究

Nucleolar organizer region associated proteins in non-Hodgkin's lymphomas

应用Ｈｏｗｅｌｌ一步法嗜银染色技术，观察１８例非何杰金淋巴瘤（ＮＨＬ）的核仁组成区嗜银蛋白，以比较不同恶性程度ＮＨＬ之间的区别并探讨其意义。每例均有高倍镜下随机计数２００个细胞的ＡｇＮＯＲ数并计算平均数和标准差，结果高度恶性者８．２６±１．７２／细胞，中度恶性者５．５１±０．９１／细胞，低度恶性者２．８２±０．５７／细胞。高度恶性的ＡｇＮＯＲ计数最高，与中度、低度恶性者之间相差非常显著（Ｐ＜０．０１）。中度恶性与低度恶性者之间ＡｇＮＯＲ计数相差也非常显著（Ｐ＜０．０１）。结果提示：ＡｇＮＯＲ检测可以作为ＮＨＬ恶性程度的一项有参考意义的指标。

Using Howell's one-step method we studied the AgNOR counts of non-Hodgkin's lymphoma (NHL) in 18 cases (6 high grade, 8 medium and 4 low grade) in order to observe the difference among the groups of high, medium and low grade of the disease. The number of AgNOR in 200 cells of each sample was counted and the mean number,standard deviation of AgNOR counts are as follows: high grade 8.26±1.72/cell,medium grade 5.51±0.91/cell,and low grade 2.82±0.57/cell. The AgNOR count of high grade NHL is the highest. A significant difference between high and medium, high and low, medium and low grade NHL(P<0.01) was observed. The findings suggest that AgNOR counts may be helpful for differentiating malignancy grade of NHL.