蛋白激酶C抑制剂抑制白细胞介素1对骨细胞的作用

Inhibitory effect of protein kinase C inhibitor on interleukin-1 activities in mouse calvarial cell cultures

用富含成骨细胞的小鼠头颅骨细胞测试白细胞介素１（ＩＬ－１）对骨细胞的作用以及蛋白激酶Ｃ（ＰＫＣ）抑制剂Ｈ－７对ＩＬ－１作用的影响。结果表明ＩＬ－１（２５～１００Ｕ／ｍｌ）呈剂量依赖方式抑制骨细胞增殖和碱性磷酸酶活性；而对耐酒石酸酸性磷酸酶活性有增强作用。Ｈ－７（５～２０μｍｏｌ／Ｌ）对ＩＬ－１上述作用具有剂量依赖抑制作用。提示ＩＬ－１对骨细胞的作用可能与ＰＫＣ有关。

Neonatal mouse osteoblast-enriched calvarial cells were used to study the effects of interleukin1(IL-1) and the possible involvement of protein kinase C(PKC) in the IL-1 action. IL-1(25～100 U/ml) significantly inhibited bone cell proliferation and alkaline phosphatase activity but stimulated tartrate-resistant acid phosphatase activity. The IL-1 induced actions were blocked by simultaneous addition of PKC inhibitor, 1-(5-isoquinoline-sulfonyl)-2-methylpiperazine(H-7,5～20 μmol/L ).These results suggest that the effects of IL-1 on bone cells may be related to PKC.