大肠癌旁粘膜细胞的PCNA和AgNORs表达观察

Observation of proliferating cell nuclear antigen and AgNORs expression in the adjacent mucosa of colorectal cancer

用增殖细胞核抗原（ＰＣＮＡ）和核仁组成区嗜银蛋白（ＡｇＮＯＲｓ）对３５例大肠癌旁粘膜进行细胞增殖表达与观察。结果表明：癌旁粘膜的ＰＣＮＡ标记指数增多，标记增强，排列紊乱。ＡｇＮＯＲｓ在癌旁粘膜中，颗粒增多、增大，形态多样。０～１ｃｍ和２～３ｃｍ组的标记指数与４～５ｃｍ组的标记指数相比较，有显著或非常显著差异（Ｐ＜０．０５或Ｐ＜０．０１）。在ＤｕｋｅＡ、Ｂ期中，细胞标记指数与Ｃ、Ｄ期相比较，两组间的标记指数有显著或非常显著差异（Ｐ＜０．０５或Ｐ＜０．０１）。提示ＰＣＮＡ和ＡｇＮＯＲｓ的标记指数增高增强，与癌的发生和患者的预后有关。ＰＣＮＡ和ＡｇＮＯＲｓ可作为临床综合治疗的一项观察指标。

cases of the adjacent mucosa in colorectal cancer were observed by the methods of PCNA and AgNORs. The results showed that the indexes of PCNA in adjacent mucosa of colorectal cancer increased. PCNA were highly labelled and expressed disorderly. The number of AgNORs granules in adjacent mucosa of cancer also largely increased and was pleomorphic. There was a significant difference between labelling index of the group of 0～1 cm or 2～3 cm and the index in the group of 4～5 cm (P<0. 05 or P<0. 01) , and between such indexes in the Dukes A.B stage and C.D stage (P<0. 05 or P<0. 01). Our results suggest that the increases of the expression of PCNA and AgNORs are related to the cancer development and the patient's prognosis. They can be recognized as clinical indicators of the cancer therapy.