PCR检测丙型肝炎病毒感染各高危人群

RT-PCR detection of HCV infection in various risk groups

用ＰＣＲ方法克隆了中国丙型肝炎病毒（ＨＣＶ）感染者的５＇端非编码区（５＇－ｎｏｎｃｏｄｉｎｇｒｅｇｉｏｎ，５＇－ＮＣＲ）序列，并根据此序列合成引物检测我国ＨＣＶ各高危感染人群的病毒复制情况，结果显示ＨＣＶＲＮＡ在慢性丙型肝炎患者的血清标本中的阳性率为８１．８％（９／１１），在ＨＣＶ抗体阳性的血液透析患者血清标本中的阳性率为６３．６％（７／１１），在ＨＣＶ抗体阳性的献血员血清标本中的阳性率为２０％（４／２０），在ＨＣＶ抗体阳性的肝细胞癌标本中的阳性率为１００％（５／５），在ＨＣＶ抗体阴性的肝细胞癌标本中的阳性率为３３．３％（７／２１）。表明ＨＣＶ的体内复制情况在各高危风险人群中的情况有明显差别；提示ＨＣＶ感染也是我国肝细胞癌发生的重要风险因子之一。

Anti-HCV antibody can be dectected in different HCV infection risk groups in which the HCV replication in vivo may be different.The sequence of HCV 5' -noncoding region(5'-NCR) from a Chinese case was cloned by reverse transcription and polymerase chain reaction(RT-PCR) method.Based on this sequence,we synthesized a pair of new primers for HCV RNA RT-PCR detection in Chinese cases and employed the RT-PCR assay to investigate the HCV replication in HCV infection risk groups. HCV RNA was found positive in 81.8%(g/11) serum specimens of chronic hepatitis C patients who were positive for anti-HCV,63.6%(7/11) serum specimens of anti-HCV positive hemadialysis patiens,20%(4/20) serum specimens of anti-HCV positive paid blood donors,100%(5/5) RNA specimens isolated from anti-HCV positive liver cancer cells and 33.3%(7/21) RNA specimens isolated from anti-HCV negative liver cancer cells.In conclusion, our data indicate clearly that:(1) HCV replication in vivo is different in various HCV infection risk groups;(2)HCV infection like HBV infection is one of high risk factors of HCC in China.