安定加强小鼠的促肾上腺皮质激素镇痛作用

Potentiation Effect of Diazepam on ACTH Analgesia in the Mice

将安定、印防己毒素和异烟肼注入小鼠腹腔内,采用痛阈测定和荧光分光光度法测定脑区5-HT含量的方法观察安定在ACTH镇痛中的作用。安定能显著加强ACTH镇痛作用,而印防己毒素或异烟肼可显著削弱ACTH的作用及安定对ACTH镇痛作用的影响。安定加强ACTH镇痛作用也可被纳洛酮或阿托品所取消。安定使海马、下丘脑和中-桥脑5-HT含量增加,而安定+ACTH可使三脑区5-HT含量进一步增加,印防己毒素或异烟肼则可对抗安定+ACTH使三脑区5-HT含量升高的作用。结果提示,安定加强ACTH镇痛作用,可能与加强脑内GABA系统的功能及激活脑内5-HT系统有关。阿片受体和胆硷能M-受体在此作用中起着重要作用。

Experimental methodssintraperitoneal injection of diazepam,r-aminobutyric acid (GABA) receptor antagonist (picrotoxin) and GABA synthesis inhibitor (iso-niazid) ;the pain threshold and serotonin ( 5 -HT) content in the brain are measured with spectrofluorometry.The effect of diazepam on ACTH analgesia (AA) is abstracted and the possible mechanisms of diazepam effect are studied.Results: diazepam increases the effect of AA.picroloxin and isoniazid reduce it and antagonize the analgesia effect of ACTH; diazepam increases the content of 5-HT in hipocampus,hypothalamus and midbrain;diazepam + ACTH increases,more than AC-TH or diazepam singly,the level of 5-HT in the 3 brain regions; picrotoxin and isoniazid reverse the effect of diazepam and ACTH on the content of 5-HT in the 3 brainsites; potentiation of diazepam on AA is antagonized by naloxone and atropine.