被动型Heymann肾类大鼠肾组织一氧化氮变化初探

The changes of renal nitric oxide in rats with passive Heymann nephritis

作者检测不同病期被动型Ｈｅｙｍａｎｎ肾炎（ＰＨＮ）大鼠肾组织一氧化氮（ＮＯ）的含量，并观察其与尿蛋白、血肌酐及肾脏病理的关系。结果：ＰＨＮ大鼠ＮＯ较正常大鼠明显减少，其中致病后２和３周末分别约及正常的１／２和１／３，４周末虽有回升，但仍低于正常组；其含量不仅与尿蛋白和血肌酐呈负相关，而且与肾脏病理改变的轻重相关。提示：膜性肾病大鼠肾组织内源性ＮＯ合成障碍，ＮＯ的减少对该病的发生具有重要作用。

Renal nitric oxide(NO) was measured at different stages in rats with passive Heymann nephritis(PHN) induced by injection of antiserum against renal tubular antigen.The value of renal NO significantly decreased in PHN rats as compared to normal rats(54.17±19.41 vs 106.14±25.19 μmol/g protein,P<0.01).At 2nd and 3 rd week after injection of antiserum,it arrived at half and one-third the level of normal rat,respectively.It began to rise but was still lower than that of normal rats at 4th week(P<0.05).In addition,the content of renal NO negatively correlated with serum creatinine (r=-0.798,P<0.01)and urine protein(r=-0.852,P<0.01).These results show that(1)impairment of endogenously synthesized NO exists in renal tissue from PHN rats.(2)NO may be involved in injury of kidney.