γ－射线诱发人淋巴细胞微核形成与细胞周期关系的初步研究

Preliminary study of the relationship of cell cycle with micronucleus formation of human lymphocytes induced by gamma rays

本研究用γ－射线诱发人外周血淋巴细胞微核（ＭＮ），使用细胞周期控制、ＢｒｄＵ免疫荧光染色、松胞素Ｂ（ＣＢ）阻滞胞质分裂等技术，研究人淋巴细胞微核形成与细胞周期间的定量关系。结果：①Ｇ０期和Ｇ１期淋巴细胞照射组和对照组相比，微核率（ＭＮＦ）和微核细胞率（ＭＮＣＦ）均无明显变化（Ｐ＞０．０５）。②Ｓ期和Ｇ２期淋巴细胞照射组和对照组相比，ＭＮＣＦ均明显增加（Ｐ＜０．０１）。（３）Ｍ期淋巴细胞照射组与对照组相比，ＭＮＦ与ＭＮＣＦ分别增加９０多倍和７０多倍。结论：在人淋巴细胞周期中Ｓ期和Ｇ２期可形成ＭＮ，但Ｍ期是形成ＭＮ的主要阶段。

The quantitative relationship of cell cycle with the micronucleus formation of the lymphocytes of human peripheral blood induced with gamma ray irradiation were studied with the control of cell cycle,bromodeoxyuridine(BrdU)immunofluorescent staining and blockade of cytoplasm division with cytochalasin B(CB).It was found that 1.The micronucleus frequency(MNF)and micronucleated cell frequency(MNCF)of C10 and C11 phases of the irradiated lymphocytes were not significantly different from those of the control(P>0.05);2.The MNCF of S and C12 phases of irradiated lymphocytes was significantly higher than that of the control(P<0.01)and 3.The MNF and MNCF of M phase of irradiated lymphocytes were 90 times and 70 times higher than the control respectively,These findings suggest that a part of micronuclei can be formed in S and C12 phases of human lymphocytes and M phase is the main stage of micronucleus formation in the whole cell cvcle.