肺癌DNA含量及细胞动力学初步研究

A preliminary study of cellular DNA content and cytokinetics with bronchoscopic in lung cancers

采用纤支镜技术，对２６例中心型肺癌进行刷检与活检，并用流式细胞仪测定新鲜癌组织的ＤＮＡ含量，观察细胞的增殖活力。结果提示，新鲜癌组织存在较高的异倍体，异倍体的出现多与恶性肿瘤存在有关；癌细胞的增殖活力亦显著增高，说明细胞的增殖活力直接与组织的恶性程度相关联。经纤支镜检查痛苦小，并发症少，患者易于接受。并且刷检和活检中的任何一项检查，多可获得阳性结果。与活检比纤支镜刷检操作简便、对组织损伤小，出血和并发症少，可弥补管壁浸润型肺癌不易获得活检标本之不足。具有一定的实用价值。

Twenty-six patients with central type of lung cancer were examined with fiberoptic bronchoscopy to obtain bronchial brushing and biopsy specimens with folw cytometric analysis of cellular DNA content and cellular proliferative capacity of lung cancer.It was found that higher level of DNA aneuploidy and significantly higher proliferative capacity were seen in the fresh lung cancer tissue than in the benign disease and normal lung tissues.Bronchial brushing is simple and less invasive technique resulting less tissue damage,bleeding and a few ccomplications and it is the technique of choice to examine the lung carcinoma with wall-infiltration,which is dificult to get the tissue specimens with biopsy