良恶性前列腺病变的纤维连接蛋白标记观察

Study of fibronectin marking of benign and malignant prostate diseases

应用免疫组化ＡＢＣ法对８４例前列腺癌（ＰＣ）、良性前列腺增生（ＢＰＨ）和非典型增生（ＰＡＨ）各３０例、以及１０例正常前列腺作了纤维连接蛋白（Ｆｉｂｒｏｎｅｃｔｉｎ，ＦＮ）标记。结果表明６３％的ＰＣ、６０％的ＰＡＨ、３７％的ＢＰＨ与３０％的正常前列腺上皮显不同的ＦＮ阳性；ＰＣ之癌间质与基膜的ＦＮ减少与缺失；ＦＮ在不同类型ＰＣ中的反应有差异，且主要见于大部分小腺泡癌、筛状癌、粘液腺癌及实体癌等；ＰＡＨ上皮的ＦＮ反应略低于ＰＣ，明显高于正常前列腺与ＢＰＨ，恶性上皮ＦＮ多呈弥漫性或散在成片的阳性着色，而良性上皮多呈局灶性ＦＮ反应。本研究证实了多数ＰＣ之癌细胞显ＦＮ强阳性或阳性反应，不同的反应强度可能与ＰＣ的类型和肿瘤细胞分化有关系；并提示癌间质与基膜ＦＮ降低和缺失可能为癌的浸润与转移提供了便利条件。

Eighty-four cases of prostatic carcinoma(PC),30 cases of prostatic atypical hyperplasia(PAH),30 cases of benign prostatic hyperplasia(BPH)and 10 normal subjects were marked with fibronectin(FN).It was found that the epithelium of 63%of cases of PC,60%of PAH,37%of BPH and 30%of the control were marked with different degrees of FN;FN in the tissues between PC and the basement membbrane was decreased or even disappeared;FN marking of different types of PC was different;positive FN marking was usually found in most of small acinar carcinnoma,cribriform carcinoma rnucinous adenocarcinoma and solid carcinoma of the prostate;FN positiveness of PAH was slightly lower than that in PC,but sign nificantly higher than that in BPH and the control.FN in the malignant epithelium was diffusely distributed or or in patches and in the benign epithelium was localized.Most of PC were strongly FN positive or positive.The differentiation of FN positiveness of PC might be related to the type and difference of PCs and FN positive parenchyma of PC and defects of the basement membrane facilitates the infiltration and metastasis of prostatic carcinoma.