犬心肌急性缺血再灌注过程中中性白细胞膜流动性变化

Changes of membrane fluidity of neutrophil during acute myocardialischemia and reperfusion in dog

作者观察了犬冠状动脉急性闭塞再灌注过程中中性白细胞膜流动性及血清肌酸磷酸激酶（ＣＰＫ）的变化．结果表明：冠状动脉左前降支（ＬＡＤ）阻断后，心大静脉回流血中中性白细胞膜荧光偏振Ｐ值升高，阻断前为０．２７±０．０１，阻断１５ｍｉｎ为０．２９±０．０１，中性白细胞膜流动性下降．随缺血时间延长，中性白细胞膜荧光偏振Ｐ值明显升高，阻断３０ｍｉｎ为０．３０±０．０１，阻断６０ｍｉｎ为０．３２±０．０１（与阻断１５ｍｉｎ比Ｐ＜０．０５）．再灌注后，中性白细胞膜荧光偏振Ｐ值继续升高．中性白细胞荧光偏振Ｐ值与血清ＣＰＫ浓度呈正相关（ｒ＝０．８９，Ｐ＜０．０５）．结论：心肌缺血再灌注过程中中性白细胞膜流动性降低，并与血清ＣＰＫ值密切相关．

The changes of neutrophil membrane fluidity and serum creatine phosphokinase(CPK )were ob-served during acute coronary artery occlusion and reperfusion in dog.The results were as follows: With theleft anterior descending branch(LAD)of coronary artery occluded ,the P value of fluorescence polarization ofneutrophil in great cardiac vein was increased from preoccluded 0.27±0.01 to 15 min occluded 0.29±0.01. The neutrophil membrane fluidity was lower.The P value of neutrophil fluorescence polarization was signifi-cantly augmented with prolonged LAD occlusion,P=0.30±0.01 when occluded for 30 min,P=0.32±0. 01after occluded 60 min(P<0.05 compared with 15 min occluded).The increase of P value of neutrophil fluo-rescence polarization was continued during reperfusion.The P value of neutrophil fluorescence polarizationwas positively correlated with the activity of serum CPK(r=0.89,P<0.05).Conclusion :The membranefluidity of neutrophil is decreased during acute myocardial ischemia and reperfusion.It is closely correlatedwith the activity of serum CPK.