摘要
目的：确定血管活性肠肽（ＶＩＰ）对培养大鼠肝癌细胞生长的影响．方法：采用四甲基偶氮唑盐比色法（ＭＴＴ试验）和细胞计数法．结果：ＶＩＰ对癌细胞增殖有明显刺激作用，加入１μｍｏｌ／ＬＶＩＰ后，培养的大鼠肝癌细胞的数量在第３日即显著增加，第４，５日细胞数增加十分显著（Ｐ＜０．０１）．对培养肝癌细胞生长的刺激效应随着ＶＰ浓度的增加而增强．最低有效浓度约为０．５μｍｏｌ／Ｌ．ＶＩＰ和肝癌细胞连续作用１２ｈ后，对培养癌细胞的生长刺激效应可维持数日．结论：ＶＩＰ可显著刺激体外培养的大鼠肝癌细胞的生长．
Objective:Vasoactive intestinal peptide ( VIP ) can stimulate the growth of
a variety of tumorcells, but there is no report about the effect of VIP on the growth of hepatoma
cells. The aim of this experi-ment was to confirm the effect of VIP on the growth of cultured rat
hepatoma cells. Methods:The tetrazoli-um colorimetric assay (MTT assay) and cell counting
method were used.Results:VIP stimulated the prolif-eration of the cultured rat hepatoma FSK
7902 cells obviously. The addition of 1 μmol/L VIP caused a signif-icant increase in the
number of cultured hepatoma cells on 3rd and maximal increase occurred on 4th day and5th
day(P<0.01).The growth promoting effect was greater as the concentration of VIP increased.
Thelowest effective concentration of VIP was 0.5 μmol/L. Exposure the VIP for 12 hours
followed by removal ofthe peptide resulted in sustained growth promotion for several days.
Conclusion:VLP stimulates the growthof cultured rat hepatoma cells.
出处
《第四军医大学学报》
1995年第3期169-172,共4页
Journal of the Fourth Military Medical University
关键词
血管活性肠肽
肿瘤细胞
四甲基偶氮唑盐
肝肿瘤
vasoactive
intestinal peptide
tumor cells,cultured
tetrazolium salts
liver neoplasms
