银杏苦内酯B对哮喘豚鼠气道反应性和嗜酸性粒细胞的影响

The effect of ginkgolide B on airway reactivity and eosinophil in asthmatic guinea pig

作者利用豚鼠哮喘模型观察豚鼠吸入卵蛋白（ＯＡ）或血小板激活因子（ＰＡＦ）后引起的气道反应，血浆和支气管肺泡灌洗液（ＢＡＬＦ）中溶血－ＰＡＦ（Ｌｙｓｏ－ＰＡＦ）含量和肺组织中嗜酸性粒细胞（Ｅ＿０）改变，及ｐＡＦ拮抗剂银杏苦内酯Ｂ（ＧＢ）的影响．结果提示致敏豚鼠ＯＡ激发和正常豚鼠ＰＡＦ激发后，与对照组相比，豚鼠血浆和ＢＡＬＦ中Ｌｙｓｏ－ＰＡＦ含量增高（Ｐ＜０．０１），肺组织中Ｅ＿０数增多（Ｐ＜０．０１），气道粘膜上皮损伤、脱落，支气管平滑肌收缩，气道反应性增高（Ｐ＜０．０１）；而预先给予ＧＢ，则以上病理变化明显减轻（Ｐ＜０．０１）．表明ＧＢ对实验性哮喘有明显的保护作用．

We have studied the influence of PAF antagonist,ginkgolide B,on the level of Lyso-PAF in serum and BALF,eosinophil number in the lung tissue from guuinea pig asthmatic models, provocated with ovalbu-min or PAF.The results revealed that after guinea pigs challenged with ovalbumin or PAF, compared with control group, Lyso-PAF content in plasma and BALF increased (P< 0.01 ),pulmonary airway eosinophil number increased(P<0.01),airway epithelium was injured,airway smooth muscle contracted,airway reac-tivity became high(P<0.01).If ginkgolide B was given before ovalbumin or PAF challenged,these patho-logic changes could be inhibited(P<0.01).These demonstrated that ginkgolide B may exert protective ef-fects on experimental asthma.