猪胰脏多酶联产实验研究

Laboratory research of simutaneous extraction and purification of di-verse enzymes from porcine pancreas

胰脏是提取胰岛素及多种酶的原料，为提高产率及高度综合利用，作者采用酸、碱抽提、硫酸铵分级沉淀、膜过滤、色谱分离等技术，从１ｋｇ猪胰脏中同时分离纯化酶达９种之多，而各酶的比活力及总活力分别为：胰蛋白酶１６０００ＢＡＥＥＵ／ｍｇ蛋白，２８．４×１０￣６ＢＡＥＥＵ；弹性蛋白酶１２０Ｕ／ｍｇ蛋白，６０００Ｕ；缓激肽酶４０．３８Ｕ／ｍｇ蛋白，３８７６Ｕ；胰凝乳蛋白酶２２０００ＡＴＥＥＵ／ｍｇ蛋白，１９．５×１０￣６ＡＴＥＥＵ；胰蛋白酶抑制剂２０８９Ｔ．Ｉ．Ｕ／ｍｇ蛋白，３１７２７Ｔ．Ｉ．Ｕ；羧肽酶Ａ９．５Ｕ／ｍｇ蛋白，３９８８Ｕ；羧肽酶Ｂ１５４．８Ｕ／ｍｇ蛋白，３４６７５．２Ｕ；核糖核酸酶Ａ８０ＫｕｎｉｔｚＵ／ｍｇ蛋白，８００Ｕ；脱氧核糖核酸酶１１３７．８Ｕ／ｍｇ蛋白，１０６０００Ｕ，本法经多次优选优化而成，建立了简易合理的工艺流程，为充分利用资源，降低成本，建立工业化生产开辟了新的途径．

Pancreas is a source of insulin and many enzymes. Further exploitation and increasing consumption have resulted in its shortage. Therefore, how to raise its yield and how to make a multipurpose use of it have become a significant job. In the present experiment,employing such techniques as Acid & alkaline extrac-tion,Ammonium sulfate fractional precipitation,Membrane infiltration and Chromatographic separation the authors simultaneously extracted and purified as many as 9 kinds of enzymes from 1kg of porcine pancreas. Their specific and total activities were as follows each: trypsin 16000 BAEEU/mg prot.,28.8×10￣6 BAEEU;elastase 120 U/mg prot.,6000 U; kallikrein 40 U/mg prot.,3876 U; chymotrypsin 22000 ATEE U/mg prot.,19.5×10￣6 ATEE U; trypsin- inhibitor 2089 T. I. U/mg prot.,31727 T.I. U; CpA 9. 52 U/mg prot.,3988U; CpB 154. 8 U/mg prot.,34675U;RNase A 80 kunitz U/mg prot.,800U;DNase 1137. 8 U/mg prot.,106000 U. The procedure underwent several optimizations, thus established a rational and simplified technological process,which promises a new way of full utilization of pancreas and industrial production of variety of enzymes from it at low cost.