四株抗金属肽的单克隆抗体可变区基因的克隆和序列测定

Amplification,cloning and sequencing of the variable region genes offour lines of monoclonal antibody against a metal-bound tetrapeptide

作者从化学合成的一种金属离子螫合的四肽的四株单克隆抗体的杂交瘤细胞中提取ＲＮＡ，反转录成ｃＤＮＡ。利用合成的寡核苷酸引物扩增了抗体的轻、重链可变区基因，并克隆人质粒。随机挑取阳性克隆各一个进行核苷酸序列分析。序列表明克隆的基因除一个轻链外均可编码小鼠抗体的轻、重链可变区。

Total RNA was extracted from a cultured hybridoma cell line secreting a kind of chemical syntheticmetal- bound tetrapeptide specific four stem monoclonal antibody and subjected to reverse transcription to de-rive cDNA.With pairs of synthetic oligomers as primers,variable region genes of the antibody's light andheavy chains were amplified and cloned into plasmids.Two individual positive clones were randomly pickedout for analysis of nucleotide sequence, which suggested that,with exception of a light chain,all clonedgenes could encode the variable regions of the light and heavy chains of murine antibody.