过敏性哮喘患者外周血单个核细胞CD23表达水平改变

Changes in the expression of CD23 on peripheral blood mononuclearcells from allergic asthmatics

目的：探讨ＩｇＥ低亲和力受体（ＣＤ２３）在过敏性哮喘发病中的作用，以及与ＩＬ－２受体（ＣＤ２５）和ＨＬＡ－ＤＲ的关系。方法：用ＡＰＡＡＰ法检测１５例过敏性哮喘患者急性发作期和缓解期外周血单个核细胞（ＰＢＭＣ）ＣＤ２３，ＣＤ２５和ＨＬＡ－ＤＲ的表达水平。结果：发作期ＣＤ２３，ＣＤ２５和ＨＬＡ－ＤＲ的表达明显强于缓解期；ＣＤ２３￣＋细胞率与ＦＥＶ＿１呈显著负相关（ｒ＝－０．５３２４，Ｐ＜０．０５），与ＣＤ２５￣＋细胞率和ＨＬＡ－ＤＲ￣＋细胞率分别呈显著正相关（ｒ分别为０．６６８８和０．６９７２，Ｐ均＜０．０１）。结论：ＣＤ２３可能介导过敏性哮喘的慢性气道炎症，活化ＣＤ４￣＋Ｔ细胞对其表达有上调作用。

Objective:Authprs investigated the role of CD23 in allergic asthma and the relationship betweenthe expression of CD23 and activated T-cells. Methods:APAAP was used to evaluate the expressions ofCD23, CD25 and HLA- DR on peripheral blood mononuclear cell(PBMC) from 15 patients with allergic asth-ma,when they were suffering acute attacks and when they were in remission.Results:Results showed thatthe percentages of CD23￣+, CD25￣+ and HLA-DR￣+ cells were much higher in episodic period than in remis-sion. The increased percentage of CD23￣+ cells was correlated with those of CD25￣+ and HLA-DR￣+ cells(r=0.6688 and 0.6972,respectively,P<0.01)and also with the decrease of FEV(r=0.5324,P<0. 05).Con-clusion:These results indicate that CD23 may induce the chronic airway inflammation in patients with allergicasthma,and that the activated CD4￣+T- cells may upregulate the expression of CD23 on PBMC.